Description
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF15 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. FGF15 is the FGF-19 ortholog in mice and they have the same amino acid sequence.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FGF15 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF15 and unlabeled FGF15 (Standards or samples) with the pre-coated antibody specific to FGF15. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF15 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF15 in the sample